性质
Related Categories Bacteria, Bioactive Small Molecule Alphabetical Index, C-CH, G Protein Function, G Proteins and Cyclic Nucleotides,
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Quality Level PREMIUM
assay ~95% (SDS-PAGE)
form lyophilized powder
Protein, ~5% Lowry-TCA
solubility H2O: 10 mg/mL
storage temp. 2-8°C
产品描述
Application
Cholera Toxin from Vibrio cholerae has been used as a control in cAMP (cyclic AMP) assay for enterotoxins.[1] It has been added as a supplement in cell culture media of primary tumors and epithelial cells.[2][3][4]
Biochem/physiol Actions
Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
包装
Package size based on protein content.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA
Reconstitution
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
